Kaj je PCR test za koronavirus?
A PCR test is short for polymerase chain reaction. This test is used to detect the coronavirus. The test detects the genetic material of the virus in a patient's blood. The test is ordered if doctors suspect a patient has the coronavirus.
To accurately diagnose the coronavirus, scientists at the National Institutes of Health use a specific set of diagnostics called the polymerase chain reaction (PCR). PCR is a technique that uses enzymes naturally present in the body to amplify a viral sample. This amplification process allows doctors to detect subtle changes between infected and uninfected people by examining how the virus-amplified DNA fragments respond to specific different reagents. "PCR amplifications used to diagnose human disease are unique because they use specific primers and end fragments and involve knowledge of the patient's internal anatomy," an NIH press release reads. These unique PCR diagnostics for coronavirus use a reagent called the polymerase chain reaction (PCR) to amplify DNA.
Kakšne so prednosti PCR v primerjavi z drugimi testi?
PCR, short for "polymerase chain reaction," is a powerful technique used to amplify (or make more) DNA present in tiny samples. The PCR portion of modern diagnostic tests is a standard part of antifungal drug diagnosis. It is widely used in coronavirus diagnostic laboratories because PCR can amplify the genetic material (or genetic code) of a virus or bacteria.
Zakaj uporabljati PCR namesto drugih metod diagnosticiranja virusov?
-Ker lahko pred-digitalne tehnike, kot sta tekoča kromatografija (LC) in plinska kromatografija (GC), ohranijo številne značilnosti vzorca, vključno s spremembami v DNK, ki jo je mogoče ekstrahirati. Tako se podatki ohranijo za nadaljnjo uporabo v raziskavah ali medicinskih raziskavah.
- Še ena razlika. LC in GC sta delovno intenzivna in sta lahko draga. Po drugi strani pa se PCR lahko izvaja v dnevni sobi ali bolnišnični kavarni. Poleg tega je dokaj preprost za uporabo, zaradi česar je najboljša izbira za izolacijo pozitivnih vzorcev za nadaljnje študije v kliničnem okolju.
- Ena od vrst PCR, ki je v zadnjem času prejela veliko pozornosti, je reverzna{1}}obrnjena fazna amplifikacija (RPA). Je razširitev LC, ki pomaga pospešiti hitrost in učinkovitost diagnosticiranja nekaterih bakterijskih in virusnih okužb.
